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Authors
Rolando, Justin C. https://orcid.org/0000-0001-8948-319X
Thieme, Anton https://orcid.org/0009-0002-1427-593X
Weckman, Nicole E. https://orcid.org/0000-0001-5894-4926
Kim, Nayoung https://orcid.org/0000-0002-3699-6458
de Puig, Helena https://orcid.org/0000-0002-5368-6996
Tan, Xiao
Cotnoir, Emily
Chaturvedi, Vishnu
Collins, James J. https://orcid.org/0000-0002-5560-8246
Walt, David R. https://orcid.org/0000-0002-5524-7348
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Article History
Received: 29 July 2024
Accepted: 2 December 2025
First Online: 14 January 2026
Competing interests
: J.J.C. and D.R.W. had financial interests in and served on the board of Sherlock Biosciences Corporation, a company that develops CRISPR diagnostics; Sherlock Biosciences was acquired by OraSure Technologies. D.R.W.’s interests are reviewed and managed by Mass General Brigham and Harvard University in accordance with their conflict-of-interest policies. Harvard College and Brigham and Women’s Hospital have filed Provisional Patent 63/764,169, on behalf of J.C.R., A.T. and D.R.W. related to the multiplexed single-molecule CRISPR detection described in this manuscript. The remaining authors declare no competing interests.
