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Large-scale, spatially resolved panoramic CRISPR screening in native tissue environments using Perturb-DBiT

Crossref DOI link: https://doi.org/10.1038/s41587-026-03127-y

Published Online: 2026-06-11

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Baysoy, Alev

Tian, Xiaolong

Renauer, Paul

Zhang, Feifei

Bai, Zhiliang https://orcid.org/0000-0002-3977-3057
Shi, Hao https://orcid.org/0000-0002-2796-629X
Yang, Mingyu https://orcid.org/0000-0003-0986-3825
Zhang, Dingyao

Liu, Miao https://orcid.org/0000-0003-4248-2661
Li, Haikuo https://orcid.org/0000-0003-3697-5662
Tao, Bo https://orcid.org/0000-0002-5490-4668
Enninful, Archibald

Lu, Yao

Gao, Fu

Wang, Guangchuan https://orcid.org/0000-0001-5315-1599
Zhang, Wanqiu

Tran, Thao

Patterson, Nathan Heath

Sheng, Jie https://orcid.org/0009-0006-0398-5089
Bao, Shuozhen

Dong, Chuanpeng

Xin, Shan https://orcid.org/0000-0002-4735-0935
Chen, Binfan

Zhong, Mei https://orcid.org/0000-0003-1230-0775
Rankin, Sherri

Guy, Cliff

Wang, Yan

Connelly, Jon P. https://orcid.org/0000-0002-5564-1775
Pruett-Miller, Shondra M. https://orcid.org/0000-0002-3793-585X
Wang, Daifeng https://orcid.org/0000-0001-9190-3704
Xu, Mina https://orcid.org/0000-0001-9513-245X
Gerstein, Mark B.

Chi, Hongbo https://orcid.org/0000-0002-9997-2496
Chen, Sidi https://orcid.org/0000-0002-3819-5005
Fan, Rong https://orcid.org/0000-0001-7805-8059
Funding

Funding for this research was provided by:

U.S. Department of Health & Human Services | NIH | National Cancer Institute (UH3CA257393)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (U54CA268083)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (U01CA294514)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (U54CA274509)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (R01CA245313)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (U01CA294514)

U.S. Department of Health & Human Services | NIH | National Cancer Institute (R33CA281702)

U.S. Department of Health & Human Services | National Institutes of Health (RF1MH128876)

U.S. Department of Health & Human Services | National Institutes of Health (U54AG079759)

U.S. Department of Health & Human Services | National Institutes of Health (U54AG076043)

U.S. Department of Health & Human Services | National Institutes of Health (RM1MH132648)

U.S. Department of Defense (HT94252310472)

Cancer Research Institute (CRI4964)
License Information

Text and Data Mining valid from 2026-06-11

Version of Record valid from 2026-06-11
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Article History

Received: 2 May 2025

Accepted: 10 April 2026

First Online: 11 June 2026

Competing interests

: A.B., X.T., Z.B., S.C. and R.F. are inventors of a patent application related to this work. R.F. is scientific founder and adviser for IsoPlexis, Singleron Biotechnologies and AtlasXomics. The interests of R.F. were reviewed and managed by the Yale University Provost’s Office in accordance with the University’s conflict of interest policies. W.Z., T.T. and N.H.P. are employees of Aspect Analytics. All other authors have no competing interests.

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