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Authors
Holland, Katie L. https://orcid.org/0009-0007-1183-195X
Plutkis, Sarah E.
English, Brian P. https://orcid.org/0000-0002-4037-6294
Daugird, Timothy A.
Sau, Abhishek https://orcid.org/0000-0002-5560-5360
Grimm, Jonathan B. https://orcid.org/0000-0003-0331-4200
Zheng, Qinsi
Sharma, Ankith https://orcid.org/0000-0002-0724-5444
Dave, Sandeep https://orcid.org/0000-0003-2832-2098
Schmidt, Anja https://orcid.org/0000-0002-5922-7037
Rahman, Fariha https://orcid.org/0000-0001-8014-3025
Xie, Liangqi https://orcid.org/0000-0002-6546-9622
Dong, Peng https://orcid.org/0000-0003-4946-1409
Tkachuk, Ariana N. https://orcid.org/0000-0001-8991-9643
Brown, Timothy A.
Singer, Robert H.
Liu, Zhe J. https://orcid.org/0000-0002-3592-3150
Musser, Siegfried M. https://orcid.org/0000-0002-7793-2557
Legant, Wesley R. https://orcid.org/0000-0003-1827-5371
Galbraith, Catherine G. https://orcid.org/0000-0003-0203-1487
Lavis, Luke D. https://orcid.org/0000-0002-0789-6343
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Funding
Funding for this research was provided by:
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
Howard Hughes Medical Institute (Janelia)
U.S. Department of Health & Human Services | National Institutes of Health (1DP2GM136653)
U.S. Department of Health & Human Services | National Institutes of Health (NS141768)
U.S. Department of Health & Human Services | National Institutes of Health (NS141768)
U.S. Department of Health & Human Services | National Institutes of Health (1DP2GM136653)
U.S. Department of Health & Human Services | National Institutes of Health (NS083085)
U.S. Department of Health & Human Services | National Institutes of Health (1DP2GM136653)
U.S. Department of Health & Human Services | National Institutes of Health (NS141768)
W. M. Keck Foundation
Welch Foundation (579662)
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Article History
Received: 28 February 2024
Accepted: 5 March 2026
First Online: 15 April 2026
Competing interests
: US patents 9,933,417, 11,091,643 and 12,440,581 describing azetidine-containing fluorophores and variant compositions (with inventors J.B.G., Q.Z. and L.D.L.) are assigned to HHMI. L.D.L. is a scientific cofounder and shareholder of Eikon Therapeutics. F.R. is currently employed as an Associate Editor for Nature Methods . She was not involved in the decision-making process for this paper and did not have access to confidential information pertaining to the peer review and editorial process. The authors declare no other competing interests.
