Balboni, Andrea
D’Angelo, Camilla
Collura, Nicoletta
Brusco, Simone
Di Berardino, Claudia
Targa, Altea
Massoti, Beatrice
Mastrangelo, Eloise
Milani, Mario
Seneci, Pierfausto
Broccoli, Vania
Muzio, Luca
Galli, Rossella
Menegon, Andrea http://orcid.org/0000-0002-2396-756X
Article History
Received: 2 February 2024
Accepted: 29 August 2024
First Online: 3 September 2024
Competing interests
: The authors declare no competing interests.
: GBM specimens used for molecular analysis and stem cell establishment were collected from patients with histologic diagnosis of primary GBM (WHO grade 4 glioma) after informed consent was obtained from all subjects and/or their legal guardian(s). The experimentation has been performed following the ethical standards of Declaration of Helsinki and in accordance with the protocol 01-CSC07 approved by the institutional ethical review board of San Raffaele Scientific Institute to R.G. Cortical neurons were prepared from C57BL6J mice that were maintained under pathogen-free conditions at San Raffaele Hospital mouse facility (Milan, Italy). Mice were used in accordance with the European Communities Council Directive of 24 November 1986 (86/609/EEC) and with the ARRIVE guidelines. The Ethics Review Committee approved experimental protocols according guidelines from the Italian Ministry of Health and from the Institutional Animal Care and Use Committee of the San Raffaele Scientific Institute (protocol number 372/2021-PR).