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Article History

Received: 5 August 2024

Accepted: 23 October 2024

First Online: 29 October 2024

Declarations

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: All animal experiments conducted in this study were conducted in accordance with the guidelines set forth by the Animal Committee of the Second Medical University of Shandong. These guidelines ensured the welfare and ethical treatment of the participating experimental animals. The objective of this study was to ascertain whether the huajieling virus infects local mammals. This study is significant for investigating the pathogenic role of the huajieling virus in mammals. The studies were conducted on local adult yellow cattle, with a total of 24 adult yellow cattle randomly selected for blood collection at three points in the vicinity of the mosquito sampling area. No animals died during the experiment, and all experimental operations were conducted under the supervision of professional veterinarians, with a view to ensuring the welfare and humane treatment of the animals involved. The study was evaluated and approved by the Ethics Committee of Shandong Second Medical University (approval number: 2022SDL477) and conducted in accordance with the standards set forth in the ethical code of conduct. Furthermore, the procedure for collecting animal blood was licensed by the Laizhou City Animal Husbandry Bureau, thereby guaranteeing the legitimacy and ethical soundness of the study methodology. This study is performed in accordance with relevant guidelines and regulations. All methods are reported in accordance with ARRIVE guidelines.

: The manuscript is original and has not been published elsewhere. All authors agree to the submission and have contributed to the work. There are no conflicts of interest.

: The authors declare no competing interests.