Wang, Po
Xiao, Jun
Zeng, Jin
Yang, Feng
Lin, Mingchao
Liang, Tao
Liu, Hucheng
Zhan, Haibo
Funding for this research was provided by:
National Natural Science Foundation of China (82060546)
Article History
Received: 8 January 2024
Accepted: 27 June 2025
First Online: 21 July 2025
Declarations
:
: The authors declare no competing interests.
: This subject study is reported in accordance with ARRIVE guidelines. All animal experiments were performed in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals and approved by the ethics committee of the First Affiliated Hospital of Nanchang University. The nude mice in this experiment were euthanized at the end of the experiment. After inhalation of isoflurane anesthesia for 2–3 min, the experimenter grabbed the root of the rat tail with his right hand and lifted it, placed it on the cage cover, pressed the head and neck of the rat downward with his left thumb and index finger, and grabbed the root of the rat tail and pulled it backward and upward with his right hand, resulting in cervical dislocation, and the spinal cord and brain stem were severed.
: All authors approved the final manuscript and the submission to this journal.