Kondo, Yuki https://orcid.org/0000-0002-1481-6225
Kobayashi, Ryotaro https://orcid.org/0000-0002-4433-4518
Kobayashi, Yuya https://orcid.org/0009-0004-5041-8920
Awata, Satoshi https://orcid.org/0000-0003-3254-7943
Funding for this research was provided by:
Japan Society for the Promotion of Science (22K20666, 23H03868)
the Sasakawa Scientific Research Grant (2023-5013)
the Tokyo Zoological Park Society Wildlife Conservation Fund
the Kurita Water and Environment Foundation (24H083)
Article History
Received: 7 May 2025
Accepted: 8 July 2025
First Online: 7 August 2025
Declarations
:
: The authors declare no competing interests.
: All experimental procedures were conducted in accordance with the ARRIVE guidelines54 and with the approval of Osaka Metropolitan University (Approval Number: S0092). All procedures involving animals adhered to the most recent ASAB/ABS ethical guidelines and were approved by the Animal Care and Use Committee of the Osaka Metropolitan University, Japan. Housing and experimental methodologies were developed to minimize stress in medaka. After the experiment, the fish were returned to their original tanks. We did not observe any behaviors indicative of stress.