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Investigation of the functional hot-spot residues of an enzyme by real-time monitoring of the enzymatic reaction using NMR and computational approaches

Crossref DOI link: https://doi.org/10.1038/s41598-026-35354-3

Published Online: 2026-01-13

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Sugiki, Toshihiko

Yoshida, Tomoki

Tsukamoto, Masaki

Miyanishi, Koichiro

Kagawa, Akinori

Miura, Natsuko

Ura, Tomoto

Fukazawa, Jun

Hatanaka, Yuko

Murata, Tsuyoshi

Fujiwara, Toshimichi

Kitagawa, Masahiro

Morita, Yasushi

Sakai-Kato, Kumiko

Takakusagi, Yoichi

Tanaka, Nobutada

Negoro, Makoto
Funding

Funding for this research was provided by:

Frontier Science Support Program by the Institute for Protein Research (R1-2)

Casio Science Promotion Foundation (J41-42)

Japan Society for the Promotion of Science (JP23K05654)

Japan Society for the Promotion of Science (JP21K06046)

Japan Agency for Medical Research and Development (JP20am0101072)

Ministry of Education, Culture, Sports, Science and Technology (JPMXS0120330644)

Core Research for Evolutional Science and Technology (JPMJCR1672)
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Text and Data Mining valid from 2026-01-13

Version of Record valid from 2026-02-11
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Article History

Received: 4 July 2025

Accepted: 5 January 2026

First Online: 13 January 2026

Declarations

:

: The authors declare no competing interests.

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