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Handling of spurious sequences affects the outcome of high-throughput 16S rRNA gene amplicon profiling

Crossref DOI link: https://doi.org/10.1038/s43705-021-00033-z

Published Online: 2021-06-29

Published Print: 2021-12-01

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Reitmeier, Sandra

Hitch, Thomas C A

Treichel, Nicole

Fikas, Nikolaos

Hausmann, Bela

Ramer-Tait, Amanda E

Neuhaus, Klaus

Berry, David

Haller, Dirk

Lagkouvardos, Ilias

Clavel, Thomas
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Text and Data Mining valid from 2021-06-29

Version of Record valid from 2021-06-29
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Article History

Received: 23 March 2021

Revised: 4 June 2021

Accepted: 11 June 2021

First Online: 29 June 2021

Competing interests

: TC has ongoing scientific collaborations with Cytena GmbH and HiPP GmbH and is member of the scientific advisory board of Savanna Ingredients GmbH.

: Sequencing datasets from mice either originated from a published study with an already approved protocol (Gnoto1 data) [CitationRef removed] or were derived from newly generated samples obtained during experiments approved by the Government of Upper Bavaria, Germany (Gnoto3 and 4; approval no. 55.2-1-54-2532-156-13/138-14), by the Institutional Animal Care and Use Committee at the University of Nebraska-Lincoln, USA (Gnoto2; Protocols 1215 and 1301), or by the Ministry for Environment, Agriculture, Conservation and Consumer Protection of the State of North Rhine-Westphalia, Germany (germfree samples; LANUV approval no. 81-02.04.2018.A396).