Fujii, Masayuki
Matano, Mami
Nanki, Kosaku
Sato, Toshiro
Article History
First Online: 3 September 2015
Change Date: 5 December 2018
Change Type: Correction
Change Details: The version of this paper originally published shows incorrect units for two plasmid concentrations. In the "Reagent Setup" section, the instructions for sgRNA-Cas9 plasmid should read "Adjust the concentration of each plasmid to 1 μg μl<sup>–1</sup>,” rather than "to 1 μg ml<sup>–1</sup>.” Similarly, all concentrations in the tables in Steps 49A, 49C, and 49D should be in μg μl<sup>–1</sup> instead of μg ml<sup>–1</sup>. Please note that these units have not been corrected in the PDF and HTML versions of the protocol available online.
Competing interests
: T.S. is an inventor on patents involving the organoid culture system (WO/2010/090513 and WO/2012/168930).