Funding for this research was provided by:
Spanish Ministry of Economy and Competitiveness (1 and 2) Council of Education of Junta de Castilla y León, Spain
Received: 29 July 2019
Revised: 17 January 2020
Accepted: 18 January 2020
First Online: 1 April 2020
: The authors declare no competing interests.
: This study was carried out in strict accordance with the EU Directive 2010/63/EU for animal experiments (ExternalRef removed), including the “three Rs” rule. The protocols were approved by the Committee on the Ethics of Animal Experiments of the University of Salamanca and the Department of Agriculture and Livestock, Regional Government of Castilla y León, Spain. All procedures were performed under isoflurane anesthesia, and all efforts were made to minimize suffering.