Zadeh Shirazi, Amin http://orcid.org/0000-0002-1906-9900
McDonnell, Mark D. http://orcid.org/0000-0002-7009-3869
Fornaciari, Eric
Bagherian, Narjes Sadat
Scheer, Kaitlin G. http://orcid.org/0000-0002-2103-0378
Samuel, Michael S. http://orcid.org/0000-0001-7880-6379
Yaghoobi, Mahdi
Ormsby, Rebecca J. http://orcid.org/0000-0002-4256-7481
Poonnoose, Santosh http://orcid.org/0000-0002-9486-4904
Tumes, Damon J. http://orcid.org/0000-0001-5709-857X
Gomez, Guillermo A. http://orcid.org/0000-0002-0494-2404
Funding for this research was provided by:
Neurosurgical Research Foundation
Cure Brain Cancer Foundation
Article History
Received: 23 October 2020
Revised: 16 March 2021
Accepted: 8 April 2021
First Online: 29 April 2021
Ethics approval and consent to participate
: The study was performed in accordance with the Declaration of Helsinki and approved by the Southern Adelaide Clinical Human Research Ethics Committee (#286.10, HREC/17/RAH/358 and HREC/18/SAC/16). Brain tumour tissue was obtained from the SANTB. Informed consent obtained from study participants was provided in writing. Consent forms and ethical approval to enable the collection of brain tumour tissues was provided by the Southern Adelaide Clinical (SAC) Human Research Ethics Committee (#286.10, HREC/17/RAH/358 and HREC/18/SAC/16). Brain tumour tissues were obtained from patients undergoing biopsy or resection of their brain tumour at Flinders Medical Centre in Adelaide, South Australia, Australia. SANTB specimens were de-identified before use in this study and all specimens were linked with non-identifiable, comprehensive clinical information.
: Images presented in the manuscript correspond to data derived using de-identified patient-derived samples and there are no details on individuals reported within the manuscript.
: Computational source codes along with the datasets used for image segmentation and RNA-seq analysis are provided in the Supplementary Information and have been deposited at GitHub (ExternalRef removed).
: The authors declare no competing interests.
: This work was supported by grants from the National Health and Medical Research Council of Australia [grant numbers 1067405, 1123816 to G.A.G.]; the Cure Brain Cancer Foundation [to G.A.G., R.J.O., and S.P.]; the University of South Australia and the MAWA Foundation [to G.A.G]; the Neurosurgical Research Foundation [to R.J.O., S.P. and G.A.G.]; the Cancer Council SA Beat Cancer Project [to G.A.G.]; and the Australian Research Council [FT160100366 to G.A.G.]. A.Z.S. is supported by an Australian Government Research Training Program (RTP) Scholarship. The funders had no role in study design, data collection and analysis, interpretation of data, the decision to publish or preparation of the manuscript.