Martín, Alberto http://orcid.org/0000-0003-3427-7684
Epifano, Carolina
Vilaplana-Marti, Borja
Hernández, Iván
Macías, Rocío I. R. http://orcid.org/0000-0002-4748-0326
Martínez-Ramírez, Ángel
Cerezo, Ana
Cabezas-Sainz, Pablo
Garranzo-Asensio, Maria
Amarilla-Quintana, Sandra
Gómez-Domínguez, Déborah
Caleiras, Eduardo
Camps, Jordi
Gómez-López, Gonzalo
Gómez de Cedrón, Marta
Ramírez de Molina, Ana http://orcid.org/0000-0003-1439-7494
Barderas, Rodrigo
Sánchez, Laura
Velasco-Miguel, Susana
Pérez de Castro, Ignacio http://orcid.org/0000-0002-8822-8274
Article History
Received: 23 May 2021
Revised: 27 June 2022
Accepted: 9 July 2022
First Online: 22 July 2022
Competing interests
: The authors declare no competing interests.
: All the experiments of the study were carried out in accordance with the Declaration of Helsinki. All mouse studies were performed in compliance with the institutional guidelines for the welfare of experimental animals approved by the Use Committee for Animal Care from Instituto de Salud Carlos III (ISCIII) and La Comunidad de Madrid and in accordance with the guidelines for Ethical Conduct in the Care and Use of Animals as stated in The International Guiding Principles for Biomedical Research involving Animals, developed by the Council for International Organizations of Medical Sciences (CIOMS). All the procedures used in the zebrafish experiments as well as fish care were performed in agreement with the Animal Care and Use Committee of the University of Santiago de Compostela and the standard protocols of Spain (Directive 2012-63-UE). At the final point of the experiments, zebrafish embryos were euthanized by tricaine overdose. Liver samples were obtained from patients having undergone surgical resection at the University Hospital of Salamanca, Spain. The study was approved by the Ethics Committee for Clinical Research of Salamanca (July 18, 2018) and informed written consent for the samples to be used for biomedical research was obtained from each patient. Plasma samples were obtained from the biobank of the Hospital Clínico San Carlos or provided by Hospital Universitari de Sant Joan (Tarragona, Spain) after approval of the Ethical Review Boards of these institutions. All subjects in the study gave their written informed consent to participate and all experiments were performed in accordance with relevant guidelines and regulations.