Zhao, Kun
Yang, Chuanxi
Zhang, Jing
Sun, Wei
Zhou, Bin http://orcid.org/0000-0003-0832-4002
Kong, Xiangqing http://orcid.org/0000-0001-8245-867X
Shi, Jing http://orcid.org/0000-0002-9183-9631
Article History
Received: 12 July 2021
Revised: 8 September 2021
Accepted: 24 September 2021
First Online: 13 October 2021
Competing interests
: The authors declare no competing interests.
: The heart samples of patients with MI were provided by the tissue bank of The First Affiliated Hospital of Nanjing Medical University, while the age- and sex-matched healthy control samples were procured from the brain stem dead patients of the Department of Brain Surgery. The above procedures were taken following protocols approved by the Institutional Review Board (IRB) on Human Subjects at Nanjing Medical University, China (2021-SRFA-241). Patients with hypertension, hyperlipidemia, diabetes, and other serious systemic diseases, including kidney diseases, were excluded.Six-week-old male C57BL6/J mice and male Sprague-Dawley (SD) rats (Vital River Biological Co., Ltd, Beijing, China) housing in a temperature-controlled room with a 12/12-h light/dark cycle were used to established myocardial infarction (MI) models. Besides, the rats were fed for in vivo experiments. All experiments with animals were conducted strictly following the university’s guidelines for the Care and Use of Laboratory Animals (publication No. 85-23, revised 1996; National Institutes of Health, Bethesda, MD, United States) and were approved by the Committee on the Use and Care of Experimental Animals of Nanjing Medical University (Nanjing, China).