van Lieshout, Laura P.
Stegelmeier, Ashley A.
Rindler, Tara N.
Lawder, John J.
Sorensen, Debra L.
Frost, Kathy L.
Booth, Stephanie A.
Bridges, James P.
Wootton, Sarah K. http://orcid.org/0000-0002-5985-2406
Funding for this research was provided by:
Ontario Graduate Scholarship
Vanier Canada Graduate Scholarship, Brock Doctoral Scholarship, and the Ethel Rose Charney Scholarship in the Human/Animal Bond
Canadian Lung Association (052919)
Gouvernement du Canada | Canadian Institutes of Health Research (352532)
Article History
Received: 26 February 2020
Revised: 18 September 2020
Accepted: 24 September 2020
First Online: 7 October 2020
Compliance with ethical standards
:
: The authors declare that they have no conflict of interest.
: All animal experiments were approved by the institutional Animal Care Committees at the University of Guelph, the Cincinnati Children’s Hospital Medical Center, and the National Microbiology Laboratory, in accordance with the Canadian Council on Animal Care guidelines (AUPP 3827). Six to eight-week-old female BALB/c mice were purchased from Charles River Laboratories (St. Constant, Quebec, Canada) and allowed to acclimatize 1 week prior to experimentation. All mice were healthy and criteria was established prior to ordering mice. Mice were of the same age, sex, and shipment. Thus, no further randomization was conducted aside from randomly selecting one cage per experimental group. The investigator was not blinded during the experiment as this study was analyzing expression as quantified objectively by a machine, and not subjective parameters such as survival endpoint in a disease model.