Kelch, Inken D.
Bogle, Gib
Sands, Gregory B.
Phillips, Anthony R. J.
LeGrice, Ian J.
Rod Dunbar, P.
Article History
Received: 3 August 2015
Accepted: 15 October 2015
First Online: 16 November 2015
Change Date: 4 February 2016
Change Type: Update
Change Details: A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Change Date: 4 February 2016
Change Type: Erratum
Change Details: Understanding of the microvasculature has previously been limited by the lack of methods capable of capturing and modelling complete vascular networks. We used novel imaging and computational techniques to establish the topology of the entire blood vessel network of a murine lymph node, combining 63706 confocal images at 2 μm pixel resolution to cover a volume of 3.88 mm<sup>3</sup>. Detailed measurements including the distribution of vessel diameters, branch counts, and identification of voids were subsequently re-visualised in 3D revealing regional specialisation within the network. By focussing on critical immune microenvironments we quantified differences in their vascular topology. We further developed a morphology-based approach to identify High Endothelial Venules, key sites for lymphocyte extravasation. These data represent a comprehensive and continuous blood vessel network of an entire organ and provide benchmark measurements that will inform modelling of blood vessel networks as well as enable comparison of vascular topology in different organs.
Competing interests
: The authors declare no competing financial interests.