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Article History

Received: 22 February 2023

Revised: 27 June 2023

Accepted: 18 July 2023

First Online: 28 November 2023

COMPLIANCE WITH ETHICAL STANDARDS

: The authors declare no conflicts of interest. This manuscript does not contain studies involving animals or human participants.

: Antimicrobial property is among the important probiotic attributes of lactic acid bacteria. The indicator-dependent conventional methods are generally used to detect antimicrobial activity of bacteriocin and other related compounds produced by these bacteria. The detection of antimicrobial activity using indicator-dependent methods is tedious, time- and manpower-consuming with least accuracy and reproducibility. More so, during screening, the chances of missing new/novel compounds are higher using these assays due to their dependency on the sensitivity of the indicator strains involved. Here, an indicator-independent assay has been discussed which is convenient and accurate in comparison to other methods used for detection of bacteriocin activity. The colorimetric assay consists of color transition of polydiacetylene vesicles after interaction with bacteriocin which is found to be specific and faster as compared to the conventional methods. Therefore, the colorimetric assay may be used for detection of bacteriocins and high-throughput screening of bacteriocin-producing lactic acid bacteria isolated from different natural sources.

: MKY and SKT conceptualized the idea. MKY prepared the manuscript. SKT edited and processed the manuscript for final submission.