Brusentsev, E. Yu.
Kizilova, E. A.
Igonina, T. N.
Ranneva, S. V.
Amstislavsky, S. Ya.
Article History
Received: 5 March 2020
Revised: 3 July 2020
Accepted: 8 July 2020
First Online: 4 May 2021
COMPLIANCE WITH ETHICAL STANDARDS
: <i>Conflict of interest</i>. The authors declare that they have no conflict of interests.<i>Statement on the welfare of animals</i>. When performing this study, all manipulations carried out with experimental animals, methods of anesthesia, euthanasia and animal care before and after experimental interventions were in accordance with international bioethics standards.
: E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky came up with and developed an experiment design. E.Yu. Brusentsev, T.N. Igonina, and S.V. Ranneva received preimplantation embryos, carried out their freezing-thawing, and their cultivation in vitro. E.A. Kizilova performed DAPI staining, counting the number of interphase nuclei, their fragments and metaphase plates in developing blastocysts. E.Yu. Brusentsev, T.N. Igonina, S.V. Ranneva, and S.Ya. Amstislavsky participated in data processing. E.Yu. Brusentsev, T.N. Igonina, and S.Ya. Amstislavsky participated in writing the text of the article. All authors participated in the discussion of the results.