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Article History

Received: 30 April 2021

Revised: 6 September 2021

Accepted: 7 September 2021

First Online: 29 December 2021

COMPLIANCE WITH ETHICAL STANDARDS

: Conflict of interest. The authors declare that they have no conflicts of interest. Statement of compliance with standards of research involving humans as subjects. The study was conducted in accordance with the Helsinki Declaration of the WMA in 2000 and the protocol of the Council of Europe Convention on Human Rights and Biomedicine in 1999; the experimental scheme used was approved by the Ethics Committee of the IEGM Ural Branch of the Russian Academy of Sciences (IRB00010009) on August 30, 2019. We used peripheral blood mononuclear cells (PBMC) from donors who were healthy, non-pregnant women of childbearing age ( n = 6). PBMC was obtained by centrifugation in a density gradient of “Diacoll” (Dia-M, Russia; ρ = 1.077 g/cm 3 ). CD33 + cells were obtained from PBMC suspension by the immunomagnetic separation method using MACS® technology (Miltenyi Biotec, Germany). To assess the effect of AFP on MDSC differentiation, we used an experimental model based on the induction of peripheral blood myeloid cells (CD33 + ) into the MDSC phenotype using the cytokines IL-6 and GM-CSF, where IL-6 creates a pro-inflammatory cytokine background and GM-CSF is a growth signal for myeloid cells [ ].