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Article History

Received: 5 September 2024

Accepted: 22 November 2024

First Online: 5 December 2024

Declarations

:

: The study was approved by the Local Ethical Committee for Animal Experiments in Olsztyn. The experiment was conducted according to European legislation (EFSA, AHAW/04–027). According to the Polish Act of 15 January 2015 (Journal of Laws, 2015, item 266) and the European Parliament Act of 22 September 2010 (Directive 2010/63/EU) on the protection of animals used for scientific or educational purposes. The need of informed consent was waived by the Local Ethical Committee for Animal Experiments in Olsztyn. The breeder declared to determine the day of the estrous cycle of the animals, to carry out the insemination and the transport of the animals to the slaughterhouse according to the procedures routinely used by farmers. Next, the animals were sold by the breeder to a slaughterhouse for the purpose of producing meat for human consumption. The tissue samples collected were by-products of the meat production process, so no additional consent from the owner was required for their use in research.

: Not applicable.

: The authors declare no competing interests.