Hu, Yuanri
Ding, Lanqing
Yan, Xu
Su, Chaoyang
Cheng, Fangzhou
Jiang, He
Zhang, Xiaoyu
Cui, Zhongkai
Funding for this research was provided by:
the National Key R&D Program of China (2022YFD2400101)
National Natural Science Foundation of China (32230107)
the Key Research and Development Project of Shandong Province (2023ZLYS02)
Central Public-interest Scientific Institution Basal Research Fund, CAFS (2023TD20)
Shandong Taishan Scholar Climbing Project
Article History
Received: 19 December 2025
Accepted: 17 March 2026
First Online: 19 March 2026
Declarations
:
: This research was carried out in compliance with the ethical standards outlined in the Experimental Animal Care, Ethics and Safety Inspection Form of the Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences (Approval No: YSFRI-2025017). All experimental procedures adhered to applicable guidelines and regulations and followed the ARRIVE guidelines. For humane euthanasia, tongue sole that either reached the experimental endpoint or were designated for sampling were sacrificed using an overdose of MS-222. Each fish was maintained in the solution for a minimum of 10 min after cessation of gill movement to ensure death.
: No applicable.
: The authors declare no competing interests.