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Article History

Received: 5 December 2017

Accepted: 26 September 2018

First Online: 10 October 2018

Ethics approval

: Maintenance and use of the animals was in accordance with the Guidelines of the Mexican Law of Animal Protection (NOM-062-ZOO-1999). All experiments were approved by the local Institutional Animal Care and Research Advisory Committee (CICUAL, Protocol ID 146), from Universidad Nacional Autónoma de México (UNAM) and under International Laws of ethical care and use of animals (National Research Council (U.S.) et al., 2011).The mice were allowed to adapt to the new conditions for two weeks at the Animal Care Facility Unit of Instituto de Investigaciones Biomédicas, UNAM. They were housed in an individually ventilated caging system under standard conditions (mean temperature 22 ± 2 °C, 40 ± 10% relative humidity, 12:12 h light-dark cycle (lights on 7:00–19:00 h)) on sterilized wood shavings bedding (ENVIGO). They received a commercial pelletized diet (TG rodent diet T2018S.15, ENVIGO) and water ad libitum from an automated watering system.Mice were sacrificed by CO2 (according to the AVMA Guidelines for the Euthanasia of Animals 2013).

: Not applicable

: The authors declare that they have no competing interest.

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