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Article History

Received: 1 February 2023

Accepted: 28 April 2023

First Online: 1 June 2023

Declarations

:

: Protocols related to animal use in this study were approved by the local animal research ethical committee (Uppsala djurförsöksetiska nämnd) and followed the Swedish Animal Welfare Act [Svensk författningssamling (SFS) 2018:1192], the Swedish Animal Welfare Ordinance (SFS 2019:66) and the Regulations and General Advice for Laboratory Animals (SJVFS 2019:9, Saknr L 150; permit numbers C419/12, C39/16, 5.2.18-17971/19, 5.8.18-19421/19 and 5.8.18-11551/19). All methods are reported in accordance with ARRIVE guidelines Essential 10, where relevant. Both female and male C57BL/6J mice (Taconic, Denmark) were included in the analysis. The mice were housed with littermates in approximately 501 cm² cages (maximum 5 mice in per cage). The mice were housed in a room temperature ranging between 20 and 24 °C and humidity of 45–65% on a 12-h light:dark cycle with lights on at 6 am. All animals were provided food (diet pellets, Scanbur, Sweden) and tap water <i>ad libitum</i>. All procedures were planned and executed to minimize stress, and euthanasia was performed during the light period of the light:dark cycle.

: Not applicable.

: The authors state no conflict of interest.