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Article History

Received: 21 September 2024

Accepted: 18 March 2025

First Online: 25 April 2025

Declarations

:

: All methods performed in this study including the plant tissue culture were in compliance with the relevant institutional, national, and international guidelines and legislation.

: Not applicable.

: The authors confirm that all the experimental research and in vitro studies on Nigella sativa callus, including plants sample, complied with relevant institutional, national, and international guidelines and legislation. Also, obtained licenses of tissue culture plants of Black cumin.

: The Tissue culture of Nigella sativa sampled comply with relevant institutional, national, and international guidelines and domestic legislation of Iran.

: In this study, we obtained permission to in vitro plants tissue culture if issued by the Agricultural and Natural Resources and science Ministry of Iran.

: The authors declare no competing interests.