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Article History

Received: 26 December 2024

Accepted: 16 April 2025

First Online: 24 April 2025

Declarations

:

: The study conformed to the ethical guidelines of the Declaration of Helsinki and was approved by the Ethics Committee of the Yantai Yuhuangding Hospital. The Ethics Committee of the Yantai Yuhuangding Hospital approved our study also waived informed consent because this study was retrospective, all information was anonymous, and there was no risk to the subjects.

: Unavailable.

: Not applicable.

: The authors declare no competing interests.

: Not applicable.

: False positives may occur when viral infections are detected through RT‒PCR, and it is impossible to distinguish them according to the criteria for positive interpretation in the kit instructions. Retesting all positive samples greatly increases the laboratory testing burden, and conversely, false positive results seriously interfere with clinical diagnosis and treatment. On the basis of many test results, this study established a cutoff value for sample retesting and elaborated on what kind of results can be directly reported and what kind of results need to be retested. In this way, the reporting of false positive results is minimized as much as possible, the laboratory testing burden is reduced, and timely reporting of the results of true positive samples is ensured. When humans respond to future “Disease X “, if the RT‒PCR technique is used for detection, the experience provided by this study can be directly adopted, providing a more effective guarantee for clinical testing.