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Article History

Received: 10 June 2024

Accepted: 13 May 2025

First Online: 22 May 2025

Declarations

:

: This study was approved by the institutional ethics committee of Zhuhai Hospital of Traditional Chinese and Western Medicine (Ethic code:20190412001). All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee, and the consent for participate was approved by the ethics committee.

: The case were conducted in accordance with the local legislation and institutional requirements. The participant provided her written informed consent to participate in this case. Written informed consent was obtained from the individual for the publication of any potentially identifiable images or data included in this article.

: The authors declare no competing interests.

: Raw sequencing data were preprocessed by fastp V.0.18.0; preprocessing included adaptor trimming, removal of the reads in which the N base reached a certain percentage (default length of 5 bp) and reads that contained low-quality bases (default quality threshold value ≤ 20) above a certain portion (default 40%), and sliding window trimming []. Clean reads were aligned to the hg19 genome (GRCh37) using Burrows-Wheeler Aligner V.0.7.15–r1140 with the default settings []. GenCore V.0.12.0 was used to remove duplicate reads []. Samtools V.0.1.19 was applied to generate pileup files for properly paired reads with mapping quality ≥ 60 [].