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A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

Crossref DOI link: https://doi.org/10.1186/s12896-017-0346-5

Published Online: 2017-03-17

Published Print: 2017-12

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Zeng, Fanli

Hao, Zhimin

Li, Pan

Meng, Yanan

Dong, Jingao

Lin, Yibin https://orcid.org/0000-0003-0608-7875
Funding

Funding for this research was provided by:

agricultural university of hebei (ZD201622, 31301616)

Agricultural University of Hebei (31271997)