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Article History

Received: 13 March 2023

Accepted: 17 August 2023

First Online: 23 August 2023

Declarations

:

: Not applicable.

: <b>Human Blood samples</b>. As decided by the Science Ethics Committees for the Capital region (VEK) in Denmark with protocol number H-4-2015-FSP, the study is a method development study and not a health science research study. Thus, the project is not subject to ethical permit as this is defined in Sect. 2.1 of the Committee Act. All patients who contributed samples to this project had blood poisoning (sepsis) with microorganisms in their blood (Hvidovre Hospital) and blood was collected for diagnostic purposes. The microorganisms were identified with routine blood culture. The patient samples that were meant to be discarded otherwise, were sent to Q-linea facilities (Uppsala, Sweden) as fully anonymized samples without accompanying personal information and microbial DNA was extracted from the samples. Additionally, fully anonymized blood samples were purchased from Karolinska Universitetslaboratoriet Huddinge, in order to be spiked in with bacterial and human DNA for the purposes of the study. No specific ethical permit was required according to Swedish legislation (The Ministry of Education and Cultural Affairs, SFS number 2003:460, Sect. 4) since the purchased blood samples were residual amounts of blood donations that were not used for transfusion.

: was obtained from all participants for research use of the residual amounts and the participants were not exposed to extra discomfort or blood loss. All methods were carried out in accordance with relevant guidelines and regulations.

: Mats Gulberg is an employee and shareholder of Q-linea. Mats Nilsson is a shareholder and member of the board of directors for Q-linea. The rest of the authors declare no competing interests.