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High yield purification of an isoleucine zipper-modified CD95 ligand for efficient cell apoptosis initiation and with biotin or DNA-oligomer binding domain to probe ligand functionalization effects

Crossref DOI link: https://doi.org/10.1186/s12896-025-00986-2

Published Online: 2025-07-01

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Shang, Xiaoyue

Bartels, Nina

Weck, Johann Moritz

Suppmann, Sabine

Basquin, Jérôme

Thaventhiran, Gajen

Heuer-Jungemann, Amelie

Monzel, Cornelia
Funding

Funding for this research was provided by:

Universität Stuttgart
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Article History

Received: 12 October 2024

Accepted: 28 May 2025

First Online: 1 July 2025

Declarations

:

: No ethics approval was required for the use of any of the cell lines in this study. The cell lines used in this study were Hela wild type cells and HEK293T cells, which are available from the ATCC organization (Manassas, VA, USA).

: Not applicable.

: The authors declare no competing interests.

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