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Article History

Received: 5 June 2018

Accepted: 2 January 2019

First Online: 18 January 2019

Ethics approval and consent to participate

: All samples were obtained from healthy or naturally infected animals in the field by qualifiers. Therefore, no aggressive operation was conducted against pigs for sampling purpose and no pigs were sacrificed in this study. The study used mice for generation of mAbs and those mice had to be sacrificed. The mice were euthanized by cervical dislocation. Thumb and forefinger were used to press down on the mice’s head. The other hand grabbed the mice’s tail and pulled it slightly back to the top to make the cervical spine disengage. The spinal cord was separated from the brain and the animal died immediately. There was no destruction of brain or section of major blood vessels in the neck during the process. All animal experiments were performed using protocols approved by Laboratory Animal Ethics Committee of Jinan University.

: Not applicable.

: The authors declare that they have no competing interests.

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