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High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Crossref DOI link: https://doi.org/10.1186/s12934-019-1066-4

Published Online: 2019-01-28

Published Print: 2019-12

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Li, Dandan

Fu, Gang

Tu, Ran

Jin, Zhaoxia

Zhang, Dawei http://orcid.org/0000-0001-5555-6151
Funding

Funding for this research was provided by:

Tianjin Science Fund for Distinguished Young Scholars (17JCJQJC45300)

Nature Science Foundation of Tianjin (16JCYBJC23500)

Tianjin Science and Technology project (15PTCYSY00020)

the Key Projects in the Tianjin Science & Technology Pillar Program (14ZCZDSY00058)

the Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences.

Natural Science Foundation of China (31670604, 31570303)

Natural Science Foundation of Liaoning Province of China (20180550237)

the Science and Technology Service Network (STS) Initiative of Chinese Academy of Sciences

National Key R&D Program of China (2018YFD0901001)
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Article History

Received: 27 March 2018

Accepted: 20 January 2019

First Online: 28 January 2019

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