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Enhanced genetic stability and expression control in growth-decoupled continuous two-stage E. coli fermentations using plasmid-dependent thyA auxotrophic selection

Crossref DOI link: https://doi.org/10.1186/s12934-025-02875-x

Published Online: 2025-12-26

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Simon, Florian https://orcid.org/0000-0002-5441-6402
Stargardt, Patrick https://orcid.org/0000-0003-4073-2292
Danielewicz, Natalia https://orcid.org/0000-0002-3886-3288
Weiß, Florian

Berein, Johanna

Striedner, Gerald https://orcid.org/0000-0001-8366-5195
Mairhofer, Juergen https://orcid.org/0000-0002-2686-1971
Funding

Funding for this research was provided by:

Österreichische Forschungsförderungsgesellschaft (46311684)
License Information

Text and Data Mining valid from 2025-12-26

Accepted Manuscript valid from 2025-12-26
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Article History

Received: 29 August 2025

Accepted: 14 November 2025

First Online: 26 December 2025

Declarations

:

: Not applicable.

: All authors agree to publishing this manuscript with Microbial Cell Factories.

: The authors declare the following competing interest: Two patent applications entitled “UNCOUPLING GROWTH AND PROTEIN PRODUCTION” and “CONTINUOUS BIOPRODUCTION BY DECOUPLING GROWTH AND PRODUCTION” were filed by enGenes Biotech with application number PCT/EP2016/059597 and PCT/EP2020/087354.

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