Alonso-Pérez, Verónica
Galant, Klaudia
Boudia, Fabien
Robert, Elie
Aid, Zakia
Renou, Laurent
Barroca, Vilma
Devanand, Saryiami
Babin, Loélia
Rouiller-Fabre, Virginie
Moison, Delphine
Busso, Didier
Piton, Guillaume
Metereau, Christophe
Abermil, Nassera
Ballerini, Paola
Hirsch, Pierre
Haddad, Rima
Martinovic, Jelena
Petit, Arnaud
Lapillonne, Hélène
Brunet, Erika
Mercher, Thomas
Pflumio, Françoise
Article History
Received: 11 June 2024
Accepted: 2 September 2024
First Online: 20 September 2024
Declarations
:
: Human sample collection: Umbilical cord blood (CB) samples were collected from healthy infants at Clinique des Noriets, Vitry-sur-Seine, France and Cell Therapy Department in Hôpital Saint Louis, Paris, France with informed consent of the mothers based on the declaration of Helsinki. Samplings and experiments were acknowledged by the Institutional Review Board of INSERM (Opinion number 12–079, IRB00003888).Human fetal liver (FL) and bone marrow (FBM) samples were collected after pregnancy termination (8-14w and 17-24w of amenorrhea) with informed consent of the parents in accordance to guidelines approved by the French Agence de Biomédecine (PFS18-009).Allograft bone marrow (ABM) and mobilized peripheral blood (mPB) samples were collected from healthy individuals at Necker Enfants Malades hospital and Centre de Recherche Biologique of Hôpital Saint Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France, respectively, with informed consent of the patients and samples were used for research only according to the Material Transfer Agreement VAL 08/08–081/01.Mouse experiments: All procedures were done in accordance with the recommendations of the European Community and French Ministry of Agriculture regulations (animal facility registration number: A920322) for the care and use of laboratory animals. Experimental procedures were specifically approved by the local Ethical Committee (CEEA 26: A18_105 and APAFIS #20538–2019050710555633). NOD.Cg-<i>Prkdc</i><sup><i>scid</i></sup><i>Il2rg</i><sup><i>tm1Wjl</i></sup>/SzJ (NSG) and NOD.Cg-<i>Kit</i><sup><i>W−41J</i></sup><i>Prkdc</i><sup><i>scid</i></sup><i>Il2rg</i><sup><i>tm1Wjl</i></sup>/KitW-41 J (NBSGW) mice were originally obtained from the Jackson Laboratory (Bar Harbor, Maine, USA), housed and bred in specific pathogen-free animal facilities (IRCM, CEA, Fontenay-aux-Roses, France). NOD.Cg-<i>Prkdc</i><sup><i>scid</i></sup><i>Il2rg</i><sup><i>tm1Wjl</i></sup>Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS) were housed and bred in the animal colony of Gustave Roussy Institute, France (CEEA 26: #2017122111548235_v2 and APAFIS #43692–2023053116192480 v3).
: The authors declare no competing interests.