Document is current

Article History

Received: 27 March 2023

Accepted: 11 July 2023

First Online: 10 August 2023

Declarations

:

: Archived tissue samples from female rhesus macaques (RMs) were obtained from the Tulane National Primate Research Center (TNPRC) and the University of Pennsylvania. All RMs were born maintained at TNPRC in accordance with the rules and regulations of the Committee on the Care and Use of Laboratory Animal Resources. All experiments were approved by the Tulane Animal Care and Use Committee. Monkeys were housed in pairs to promote the psychological well-being of nonhuman primates. Enrichment included manipulable items in the cages (durable and destructible objects), perches or swings, various food supplements (fruit, vegetables, primate treats), foraging or task-oriented feeding methods and human interaction with caretakers and research staff as dictated by The United States Animal Welfare Act. Enrichment devices are rotated on a weekly basis and include toys, mirrors, radios, TV/VCRs, foraging boards, and a variety of complex foraging devices. Animals were fed a normal monkey chow. Animal care staff monitored the health and well-being of the animals daily with physical examinations performed weekly. All possible measures were taken to minimize discomfort of the animals. Anesthesia and analgesics were used and administered under the direction of a licensed veterinarian. All procedures were performed under ketamine or telazol anesthesia. Analgesics such as buprenorphine and lidocaine were used preemptively and following each potentially painful procedure. All animals were sacrificed according to humane endpoints consistent with the recommendations of the American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals.

: Dr. Burdo is a member of the Scientific Advisory Board and holds equity in Excision BioTherapeutics (unrelated to this study). All other authors declare no conflicts of interest.