Document is current

Article History

Received: 14 January 2023

Accepted: 11 May 2023

First Online: 29 May 2023

Declarations

:

: We strictly followed the rules to get the Ethical Review Approval Letter No.2020DW-31-02 of Animal Ethics Committee in Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, which included the animal study of this manuscript. Animal studies were in compliance with the ARRIVE guidelines []. And all efforts were made to minimize animals’ suffering and to reduce the number of animals used. The mice were euthanized by professional technicians. We euthanize mice when their death is inevitable, including experimental needs and life needs. Under the guidance of professionals, the mice to be euthanized were placed in a sealable euthanasia device, and CO₂ was released to suffocate the experimental animals. The mice were first placed in the induction phase and provided with air, then the CO₂ concentration was continuously increased until respiratory cardiac arrest occurred. Each mouse had its own confined space. The whole experiment was guided and evaluated by professionals. All authors and experimental participants know and agree with the animal experiments involved in this paper, and strictly abide by the animal ethics requirements.

: Not applicable.

: The authors declare that there are no competing intereststs.