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Article History

Received: 2 July 2024

Accepted: 18 December 2024

First Online: 4 January 2025

Declarations

:

: Not applicable.

: This study was financially supported by Pharmacosmos A/S (Holbaek, Denmark). Author Wesley Lyons was employed by the company Pharmacosmos A/S. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Pharmacosmos A/S was neither involved in performance of study, data evaluation or data interpretation.

: Pigs were housed in accordance with the German regulations on pig husbandry and the national animal welfare law. Husbandry conditions and the animal trial procedure follow regulations of the European Convention concerning the protection of vertebrate animals and followed the Directive of the European Convention for the Protection of Vertebrae Animals used for Experimental and Other Scientific Purposes (European Treaty Series, nos. 123). The study was approved from the local permitting authorities in the Lower Saxony State Office for Consumer Protection and Food Safety and in accordance with the requirements of the national animal welfare law (approval number: 33.19-42502-04-22-00132) following the guidelines of FELASA and ARRIVE. The animals were handled according to high ethical standards and national legislation. Unnecessary suffering was avoided by precautions taken at all stages of the experiment, as e.g. blood sampling was performed by an experienced and skilled veterinarian. Pigs were continuously observed during the whole experiment. Humane intervention points were defined for circulatory failure during life-threatened emergency and drug intolerance situations. Decisions for euthanasia due to diseases were based on medical and ethical considerations []. Euthanasia was conducted by injection of 60 mg pentobarbital/kg bw intravenously. All animal procedures were approved by the Lower Saxony State Office for Consumer Protection and Food Safety.

: Data have not been published previously.