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Authors
Ma, Jingchun
Jin, Wei
Rong, Li
Gao, Zhanyu
Hazrat, Zaman
Shakhawat, Hosen Md
Long, Fei
Zhang, Zixuan
Huang, Jiandong
Lu, Xiaomei
Jin, Guoxiang
Zhou, Zhongjun
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Funding
Funding for this research was provided by:
University Grants Committee (T13-602/21N)
High-level Hospital Construction Project of Guangdong Provincial People's Hospital (KJ012019517)
Guangdong-Dongguan Joint Research Scheme Guangdong-Hong Kong-Macau Program (2021B1515130004)
Science, Technology and Innovation Commission of Shenzhen Municipality (JCYJ20210324 114408024)
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Article History
Received: 9 September 2024
Accepted: 19 June 2025
First Online: 7 July 2025
Declarations
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: All experimental procedures involving animals were conducted in accordance with institutional ethical guidelines and regulatory standards under approval from the Committee on the Use of Live Animals for Teaching and Research (CULATR) at the University of Hong Kong (Animal License No. [25–144] in DH/HT&A/8/2/3 Pt.80).
: Not applicable.
: W.J., J.M., Z.Z. and L.R. declare that a patent application related to the IT-scC&T-seq technology is in preparation. The intended application will cover the indexed tagmentation and barcoding strategy described in this study. This does not restrict academic or non-commercial use of the method. All experimental protocols and relevant details are fully described in the manuscript and supplementary materials, allowing full reproducibility. Researchers are free to use and implement the method for non-commercial purposes without requiring a license. Any future commercial use may require appropriate licensing arrangements. The remaining authors declare no competing interests.
