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Article History

Received: 3 November 2020

Accepted: 11 January 2021

First Online: 24 February 2021

Ethics approval and consent to participate

: Laboratory rats were maintained and used for blood feeding of <i>Ae. aegypti</i> and <i>C. quinquefasciatus</i> colonies under Animal Use Protocol A2018 02-002-Y1-AO, which was approved by The University of Georgia Institutional Animal Care and Use Committee (IACUC). The UGA IACUC oversees and provides veterinary care for all campus animal care facilities and is licensed by the US Department of Agriculture (USDA) and maintains an animal welfare assurance, in compliance with Public Health Service policy, through the NIH Office of Laboratory Animal Welfare, and registration with the USDA APHIS Animal Care, in compliance with the USDA Animal Welfare Act and Regulations, 9 CFR. IACUC personnel attend to all rodent husbandry under strict guidelines to insure careful and consistent handling. The University of Georgia’s animal use policies and operating procedures facilitated compliance with applicable federal regulations, guidance, and state laws governing animal use in research and teaching including the: (i) Animal Welfare Act, (ii) Public Health Service (PHS) Policy on the Humane Care and Use of Laboratory Animals, (iii) United States Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research and Training, (iv) Guide for the Care and Use of Laboratory Animals, (v) Guide for the Care and Use of Agricultural Animals in Research and Teaching, (vi) American Veterinary Medical Association Guidelines for the Euthanasia of Animals and (vii) Applicable Georgia laws. No human subjects or tissue were used in this study.

: Not applicable.

: The authors declare that they have no competing interests.