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Article History

Received: 16 October 2017

Accepted: 23 February 2018

First Online: 20 March 2018

Ethics approval and consent to participate

: Two healthy individuals were vaccinated with the 2016 Fluarix quadrivalent seasonal influenza vaccine. Vaccinated individuals who participated in this study provided informed consent in writing in accordance with the protocols approved by the IRB of Emory University IRB#00089789, entitled “sc-RNA-seq for clinical samples.” Peripheral blood CD19+ B cells were obtained from a healthy, unvaccinated individual who provided informed consent and was recruited under the auspices of Emory IRB#00045821, entitled “Phlebotomy of healthy adults for the purpose of evaluation and validation of immune response assays.” These protocols adhere to international guidelines established in the Declaration of Helsinki by the World Medical Association.All rhesus macaque samples were obtained from animals undergoing vaccine studies housed at the Yerkes National Primate Research Center, which is accredited by the American Association of Accreditation of Laboratory Animal Care. This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health, a national set of guidelines in the USA, and also to international recommendations detailed in the Weatherall Report (2006). This work received prior approval by the Institutional Animal Care and Use Committees (IACUC) of Emory University (IACUC protocol #YER-2002353-061916GA, entitled Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery-Parent Project, and #2000936, entitled B-cell Biology of Mucosal Immune Protection from SIV Challenge. Appropriate procedures were performed to ensure that potential distress, pain, discomfort, and/or injury were limited to that unavoidable in the conduct of the research plan. The sedative ketamine (10 mg/kg) and/or tiletamine/zolazepam (Telazol, 4 mg/kg) was applied as necessary for blood draws, and analgesics were used when determined appropriate by veterinary medical staff.

: Not applicable.

: The authors declare that they have no competing interests.

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