Bessa-Andrês, Catarina
Pinto-Cardoso, Rui
Tarasova, Karyna
Pereira-Gonçalves, Ana Luísa
Gaio-Ferreira-Castro, Joana Maria
Carvalho, Liliana S.
Costa, Maria Adelina
Ferreirinha, Fátima
Canadas-Sousa, Ana
Marinhas, José
Freitas, Rolando
Lemos, Rui
Vilaça, Adélio
Oliveira, António
Correia-de-Sá, Paulo http://orcid.org/0000-0002-6114-9189
Noronha-Matos, José Bernardo
Funding for this research was provided by:
Fundação para a Ciência e a Tecnologia (UIDB/04308/2020, UIDP/04308/2020, EXPL/MED-FAR/1065/2021, SFRH/BD/144613/2019, SFRH/BD/135942/2018, COVID/BD/153196/2023)
Article History
Received: 27 February 2024
Accepted: 27 May 2024
First Online: 18 June 2024
Declarations
:
: Not applicable.
: Informed consent to use the biological material that would be otherwise discarded was obtained. All procedures were approved within the scope of the project “BONE-PURI(NO)AGEING – Regeneration of aged human bone through purinome activation in mesenchymal stem cells – preclinical studies”, followed by the project “BONEREGENERA - New insights of the purinome in predicting bone healing by mesenchymal stromal cells in osteoporotic patients” by the Ethics Committees of Centro Hospitalar de Vila Nova de Gaia – Espinho (registration nº 137/2018-2, endorsed on January 10, 2019) and of Gabinete Coordenador de Investigação / DEFI – Centro Hospitalar Universitário de Santo António (CHUdSA, registration nº 2021-002(001-DEFI-001-CE, endorsed on September 01, 2021), and of Instituto de Ciências Biomédicas Abel Salazar (Medical School) of the University of Porto. The investigation conforms to the principles outlined in the Declaration of Helsinki. Animals care and experimental procedures were conducted in strict accordance with the recommendations of the European convention for the Protection of Vertebrate animals used for experimental and other Scientific purposes (ETS 123), Directive 2010/63/EU, and Portuguese rules (DL 113/2013). All experimental protocols involving animals were approved by the competent national authority Direção Geral de Alimentação e Veterinária and by the ICBAS Animal Ethical Committee (No. 224/2017); experimental design, data analysis, and reporting are in accordance to Curtis, Ashton, Moon, and Ahluwalia. []. All efforts were made to minimize animal suffering and to reduce the number of animals in compliance with the ARRIVE 2.0 guidelines Percie Du Sert et al. []. This exploratory study was not pre-registered.
: The authors have declared that no conflict of interest exists.