Ito, Ryoji http://orcid.org/0000-0003-2903-2332
Ohno, Yusuke
Mu, Yunmei
Ka, Yuyo
Ito, Shuko
Emi-Sugie, Maiko
Mochizuki, Misa
Kawai, Kenji
Goto, Motohito
Ogura, Tomoyuki
Takahashi, Riichi
Niwa, Akira
Nakahata, Tatsutoshi
Ito, Mamoru
Funding for this research was provided by:
Japan Society for the Promotion of Science (#25430099, 18H03975)
Article History
Received: 17 August 2023
Accepted: 11 June 2024
First Online: 21 June 2024
Declarations
:
: All animal experiments were approved by the Institutional Animal Care and Use Committee of the CIEM (certification number: AIA230021-001) and were performed in accordance with the CIEM guidelines. The title of approved project is “Development of immunodeficient mice and application to humanized model animals”, and date of approval is Feb. 22, 2023. All experiments that employed human-derived cells were approved by the Institutional Ethics Committee of the CIEM (certification number: 19 − 11) and performed in accordance with the CIEM guidelines. The title of approved project is “Application of In Vivo Experimental Models with Human Cells (Humanized Mice) in Biomedical Research -- In Vivo Characterization of Human Umbilical Cord Blood Cells, Somatic Cells, and Tumor Cells --”, and date of approval is Nov. 1, 2019. In accordance with our IACUC guidelines, pain relief during blood collection and transplantation procedures was provided by the inhalation of 2% isoflurane anesthesia. The euthanasia protocols adhered to IACUC-approved methods, including cervical dislocation or excessive inhalation of isoflurane. For human cell transplantation experiments, the experimental period was capped at a maximum of 30 weeks to ensure compliance with IACUC-approved timeframes. In cases where animals exhibited severe anemia or symptoms of autoimmune disorders, humane endpoints were applied, as mandated by IACUC guidelines. These procedures were meticulously implemented to uphold the ethical treatment and welfare of the animals throughout the study, in strict accordance with the IACUC regulations and principles.
: All the experiments in this work have been reported in line with the ARRIVE guidelines 2.0.
: Not applicable.
: The authors declare no competing interests.