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Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community

Crossref DOI link: https://doi.org/10.1186/s40104-025-01291-w

Published Online: 2025-12-01

Update policy: https://doi.org/10.1007/springer_crossmark_policy

Authors

Choi, Youyoung

Zhou, Mi

Romero-Pérez, Atmir

Beauchemin, Karen A.

Duval, Stephane

Kindermann, Maik

Guan, Le Luo https://orcid.org/0000-0002-8420-4371
Funding

Funding for this research was provided by:

Beef Cattle Research Council Cluster (FDE.18.21C)

DSM Nutritional Products

Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery, and NSERC Alliance program (Canadian Research Chair Program (Tier 1), ALLRP 588541-23)
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Article History

Received: 11 May 2025

Accepted: 12 October 2025

First Online: 1 December 2025

Declarations

:

: The experimental protocols received approval from University of Alberta Animal Care and Use Committee for Livestock (Protocol No. short-term: AUP#1132; long-term: AUP#1234). The experiments were conducted following the guidelines of the Canadian Council of Animal Care (2011, Ottawa, ON, Canada).

: All authors have consented to publication.

: There are no competing interests among authors.

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