Rajakulasooriya, R. Shashika R. https://orcid.org/0009-0000-4327-2493
Fernando, S. S. Neluka
Gunasekara, T. D. Chinthika P.
Jayaweera, Pradeep M.
Kumarasinghe, K. G. Upul R.
Thabrew, H. Harshani P. M. J.
Chan, Enoch
Buddhika, R. B. J.
Weerasinghe, G. G. Yashoda H.
Karunarathna, K. A. A. Ureshani
Funding for this research was provided by:
The Open University of Sri Lanka (OUSL/FHS/23/02, OUSL/FHS/24/02)
Article History
Received: 13 December 2024
Accepted: 20 January 2025
First Online: 23 January 2025
Declarations
:
: In accordance with the Council of Europe Directive 2010/63/EU and its guidelines on the protection of experimental animals, zebrafish embryos are not considered protected organisms until they reach the stage of independent feeding (up to 5 days post-fertilization/120 hours post-fertilization hpf), and therefore, do not fall into the regulatory frameworks dealing with animal experimentation. In adhering to the regulatory frameworks outlined in EU Directive 2010/63/EU, we ceased all the experiments at 96 hpf. As such, ethical approval was not required for the fish embryo toxicity experiment procedures conducted herein. Zebrafish embryos were maintained and used in accordance with all the international ethical guidelines and institutional standards. For this type of study informed consent is not required.
: Not applicable.
: The authors declare no competing interests.