Aumailley, Lucie
Bodein, Antoine
Adjibade, Pauline
Leclercq, Mickaël
Bourassa, Sylvie
Droit, Arnaud
Mazroui, Rachid
Lebel, Michel http://orcid.org/0000-0002-3499-7174
Funding for this research was provided by:
Canadian Institutes of Health Research (PJT-173398)
Natural Sciences and Engineering Research Council of Canada (MOP-CG095386)
Article History
Received: 24 November 2023
Accepted: 25 April 2024
First Online: 12 May 2024
Declarations
:
: <i>Gulo</i><sup><i>−/−</i></sup> mice were obtained from the Mutant Mouse Regional Resource Centers (University of California Davis, CA) and housed at the Centre Hospitalier de l’Université Laval animal facility and maintained with 0.4% (weight/volume) of L-ascorbate (vitamin C; Sigma-Aldrich, Oakville, ON) in drinking water. <i>Gulo</i><sup><i>−/−</i></sup> mice were backcrossed onto the C57BL/6NHsd background (Harlan Laboratories, Frederick, MD, USA) for 12 successive generations. Finally, heterozygous mice were crossed to obtain <i>Gulo</i><sup><i>−/−</i></sup> and wild type (<i>Gulo</i><sup><i>+/+</i></sup>) mice. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Canadian Council on Animal Care in science and the protocol was approved by the Committee on the Ethics and Protection of Animal of Laval University (permit Number: CHU-22-1039). All mice were fed ad libitum with Teklad Global 18% protein rodent diet, 6% fat, 110 IU/kg of vitamin E, and 15 IU/g of vitamin A (Envigo cat. # 2918, Madison, WI, USA).
: Not applicable.
: The authors declare that they have no competing interests.