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Article History

Received: 30 December 2022

Accepted: 9 February 2023

First Online: 1 March 2023

Declarations

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: Female BALB/c mice aged 6–10 weeks (<i>n</i> = 18) were obtained from SLC (Japan). The total number of mice was determined from the results of previous experiments. (Calculate the mean and standard deviation, and calculate the number of samples for which a significant difference can be determined.) Mice were housed in 3 per cage. The mice were raised under the following conditions: 12 h light/dark cycles, temperature of 23 + 2 °C, and relative humidity of 55%, at the Nagasaki University Animal House Facility; food and water were provided freely. The mice were randomly divided into three groups: control (<i>n</i> = 6), benznidazole (<i>n</i> = 6) and coptisine (<i>n</i> = 6) groups. All experiments were approved and performed according to the guidelines of the Care and Use of Laboratory Animals of the University of Nagasaki (approval number R12005). Mice were observed daily during the experiment to check for rapid weight loss and sudden mouse death. Prior to being subjected to experimentation or killing, the mice received isoflurane inhalation to minimize suffering. All methods were reported in accordance with the ARRIVE guidelines ().

: Not applicable.

: The authors declare that they have no competing interests.