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Article History

Received: 25 July 2025

Accepted: 26 October 2025

First Online: 27 January 2026

Declarations

:

: Cell collection for this study was conducted with the approval of the Ethics Committee of the School of Dentistry, Aichi-Gakuin University.Title: Establishment of a method for mass culture of dental pulp and periodontal ligament stem cells using a gravity-controlled culture system.(Approval No.: AGUD621 Approval Date: 2021.3.25).The research plan was disclosed, including the voluntary nature of research cooperation, research objectives, and research methods. The benefits and disadvantages to participants, publication of research results, data handling, and cost burdens were also documented and explained in advance in an easy-to-understand manner. The fact that consent could be withdrawn even after the start of the research was clearly stated in the explanatory document, and if a participant refused to participate in the research or requested to discontinue participation, the research was immediately suspended and the opportunity to withdraw consent was guaranteed. After ensuring full understanding, we obtained signatures on the consent form. For subjects under 18 years of age, we obtained consent from a legal representative. For subjects who were minors but 16 years of age or older, we also obtained the subject's own consent. For subjects under 16 years of age but 6 years of age or older, we endeavored to obtain assent from the subject as well.In addition, all animal experiments in this study were conducted in accordance with the Regulations for the Conduct of Animal Experiments of the School of Dentistry, Aichi-Gakuin University.Title: Development of a Novel Living Post-Pulp Amputation Drug.(Approval No.: AGUD499 Approval Date: 2023.5.24).

: Not applicable.

: The authors declare that they have no competing interests.