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Article History

Received: 18 May 2020

Accepted: 3 May 2021

First Online: 30 May 2021

Declarations

:

: The protocol of this study was approved by the research ethical approval committee of the Medical Research Center, Ain Shams University, Cairo, Egypt. The Guidelines and Principles of laboratory animal care (NIH publication No. 85-23, revised 1985) were followed, as well as specific national laws where applicable. The living conditions of animals were appropriate for their species and contribute to their health and comfort (the housing, feeding, and care of all). The authors ensured that all individuals who deal with animals were under their supervision and received explicit instruction in experimental methods and in the care, maintenance, and handling of the rats. The authors were refining the experimental procedures themselves. They were carefully assessing the method of administration, the effects of the substance on the animal, and the amount of handling and restraint required. All rats were sacrificed using euthanasia to prevent the animals from feeling pain.

: Not applicable

: Authors have declared that no competing interests exist.