Funding for this research was provided by:
Fondazione Telethon (GGP17178)
Text and Data Mining valid from 2019-11-15
Received: 25 July 2019
Accepted: 29 October 2019
First Online: 15 November 2019
Ethics approval and consent to participate
: Oocytes harvesting from <i>Xenopus laevis</i> frogs was performed according to the Italian Government Legislation on the ethical treatment of animals. After surgery, frogs were allowed to recover from anesthesia and suitable aftercare was given. Frogs were used two or three times for oocyte harvesting allowing for at least 2 months of recovery between the operations. After the final operation, frogs were deeply anaesthetized and then killed by decapitation. The anesthetic used was tricaine (ethyl 3-aminobenzoate methanesulfonate salt, Sigma) at a concentration of 1.5 g/l buffered to neutral pH with sodium bicarbonate.
: Not applicable
: The authors declare no potential financial and non financial conflicts of interest with respect to this research.