Funding for this research was provided by:
Ministry of Science and Technology, Taiwan (MOST 104-2628-E-006-004-MY3)
Text and Data Mining valid from 2018-05-09
Received: 7 February 2018
Accepted: 25 April 2018
First Online: 9 May 2018
Ethics approval and consent to participate
: This work prepared 32 zebrafish (Danio rerio, AB strain) larvae that were treated with 0.003% 1-phenyl 2-thiourea (PTU) at 24-h post-fertilization (hpf) to prevent melanogenesis. At 3 dpf, the zebrafish larvae were placed on concavity slides and mounted in 3% methylcellulose without anesthesia for recording. The zebrafish maintenance followed the standard husbandry procedures [CitationRef removed]. All the zebrafish experiments were approved by the Affidavit of Approval of Animal Use Protocol of National Cheng Kung University (IACUC Approval NO. 96062). The larval cardiac videos were recorded by Leica Z16 APO microscope equipped with Canon EOS 550D megapixel digital single-lens reflex camera. The videos used for analysis were recorded in 60 frames per second (fps) with a resolution of 1280 X 720.
: The authors declare that they have no competing interests.