Funding for this research was provided by:
Agence Nationale de la Recherche (ANR-13-LAB3-0008-01, ANR-11-INBS -0003)
Text and Data Mining valid from 2018-05-29
Received: 12 September 2017
Accepted: 7 May 2018
First Online: 29 May 2018
Ethics approval and consent to participate
: All study methods were implemented in accordance with EU guidelines and regulations (Directive 2010/63/UE). For animals maintained in INRA facilities, the experimental protocols were approved by the INRA local Ethics Committee (COMETHEA, authorization numbers 12/160 and Méjusseaumes Animal Care committee 0162503). Mice semen samples were collected from 7-week-old male C57Bl/6JOlaHsd mice supplied by Harlan Laboratory (Venray, Netherlands) and euthanized by cervical dislocation. The bull samples were provided by commercial companies: Montbéliarde breed by GEN’IATEST (Roulans, France) and UMOTEST (Ceyzeriat, France), Holstein and Normande breeds by EVOLUTION (Rennes, France) and Belgian White Blue Breed by AWE (Ciney, Belgium); the ram, goat and boar semen were also supplied by commercial companies, Oson (France), CAPGENES (France) and Laboratoire National de Contrôle des Reproducteurs (LNCR, France), respectively. Stallion semen was supplied by Dr. M. Magistrini (UMR INRA 0085 PRC, Nouzilly, France). Human sperm samples from patients included in a PHRC METASPERME study, coordinated by Dr. R. Levy (Laboratoire d’Histologie Embryologie Cytogénétique CECOS, Hôpital Jean Verdier, Bondy, France); this study received the approval from French ethics board (Conseil d’évaluation éthique pour les recherches en santé, CERES) and all the patients gave their informed written consent to participate.
: The authors declare that they have no competing interests.
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